Soroka A.I.

Influence of nutrient medium composition on callusogenesis and regeneration in anther culture of oil flax

Cytology and Genetics 2004, v 38, number 2, 20-25

Regeneration ability in callus cultures from anthers of two hybrid genotypes of oil flax was studied on N₆ and LMA-1 nutrient media at various concentrations of cytokinine 6-benzylamynopurine (BAP). It was shown that callus grew and developed better at BAP concentrations of 2mg/l, comparing with 4 and 6mg/l. Shoot and root regeneration was observed in F₁ genotype 6-8-gnezdny × M22 only and did not depend on BAP concentration in the medium and on the medium composition itself. Transfer onto fresh medium often stimulated dedifferentiation of the regenerated structures.