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Variability of nuclear 18S-25S rDNA of Gentiana lutea L. in nature and in tissue culture

Melnik V.V., Spiridonova K.V., Andreev I.O., Strashnyuk N.M., Kunakh V.A.

 


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18S-25S rDNA sequence in genomes of G.lutea plants from different natural populations and from tissue culture has been studied with blot-hybridization method. It was shown that ribosomal repeats are represented by the variants which differ for their size and for the presence of additional HindIII restriction site. Genome of individual plant usually possesses several variants of DNA repeats. Interpopulation variability according to their quantitative ratio and to the presence of some of them has been shown. Modifications of the range of rDNA repeats not exceeding intraspecific variability were observed in callus tissues in comparison with the plants of initial population. Non-randomness of genome modifications in the course of cell adaptation to in vitro conditions makes it possible to some extent to forecast these modifications in tissue culture.

Tsitologiya i Genetika 2004, vol. 38, no. 3, pp. 16-21



Melnik V.V., Spiridonova K.V., Andreev I.O., Strashnyuk N.M., Kunakh V.A. Variability of nuclear 18S-25S rDNA of Gentiana lutea L. in nature and in tissue culture, Tsitol Genet., 2004, vol. 38, no. 3, pp. 16-21.




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