Sytnik E.S., Parij A.F., Komarnitzki I.K., Gleba Yu.Yu., Kuchuk N.V.

Analysis of nuclear and mitochondrial genomes of transplastomic Salpiglossis sinuata plants obtained by transfer of transformed plastids from N. tabacum (+S. sinuata) cybrid

Cytology and Genetics 2003, v 37, number 5, 3–8

New model system of plastid transformation has been proposed using a wild representative of Solanaceae family – S. sinuata. Earlier obtained cybrid plants N. tabacum (+S. sinuata) were used for transformation experiments by PEG treatment of protoplasts with aadA gene that confers resistance to spectinomycin. Transformed  S. sinuata plastome was transferred from N. tabacum (+S. sinuata) cybrid to S. sinuata wild type plants by somatic hybridization. Molecular analysis of nuclear and mitochondrial DNA has been performed.