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Vasilenko M.Yu., Komarnitskyy I.K., Sakhno L.A., Gleba Yu.Yu., Kuchuk N.V.
Obtaining and the Analysis of Intergeneric Somatic Hybrids between Brassica napus and the “Albino” Line of Orychophragmus violaceus
Cytology and Genetics 2003, v 37, number 1, 3–10
The Orychophragmus violaceus chlorophyll-defective line of “albino” type has been obtained by spectinomycin treatment. Somatic hybridization between Orychophragmus violaceus and Brassica napus was performed by fusion of green mesophyll protoplasts of rape and callus protoplasts of the O. violaceus “albino” line. Near two hundred of regenerant plants were selected according to the regeneration type and ability to become green, and were determined as hybrids. Chloroplast DNA in selected hybrids was identical to rape chlDNA, which was confirmed by the PCR-RFLP analysis of plastid DNA fragments. Fragments of hybrid mitochondrial DNA analyzed by the PCR-RFLP analysis were identical to fragments of O. violaceus. The nuclear genome of the majority of hybrids was represented by the O. violaceus genome, which was demonstrated by analyses of isoenzymes, DNA telomeric sequences, ribosomal and satellite DNAs, and the RAPD analysis. The cytogenetic analysis of a number of lines has shown variability in the number of chromosomes in the obtained lines.
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