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Study of the intracultivar variability of grape DNA using RFLP and PCR method

Barysheva I.A., Tulaeva M.I., Chisnikov V.S.

 


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Evaluation of DNA polymorphism among Vitis vinifera varieties using RFLP and PCR methods has been performed to choose a DNA technology for detection of grape intracultivar variation. DNA polymorphism of clones of the varieties Riparia Gluar, Riparia ? Rupestris 101-14, Cabernet Sauvignon, Ristling reinskiy has been studied using Southern hybridization and amplification techniques. It has been shown that grape intracultivar variability of rDNA in Riparia ? Rupestris 101-14 and Cabernet Sauvignon clones was caused by the modification in AluI restriction sites of rDNA. DNA variability of the randomly amplified and inter-SSR sequences of the Riparia Gluar, Riparia ? Rupestris 101-14, Cabernet Sauvignon clones was also detected. A set of molecular DNA loci which can be used for grape clone identification has been obtained

Tsitologiya i Genetika 2003, vol. 37, no. 6, pp. 31-38



Barysheva I.A., Tulaeva M.I., Chisnikov V.S. Study of the intracultivar variability of grape DNA using RFLP and PCR method, Tsitol Genet., 2003, vol. 37, no. 6, pp. 31-38.




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