Mitrofanova I.V., Galaev A.V., Sivolap Yu.M.

Investigation of molecular-genetic heterogeneity of clematis plants (Clematis l.) obtained via organogenesis and somatic embryogenesis in vitro

Cytology and Genetics 2003, v 37, number 6, 12-16

Genome variability of in vitro micropropagated Clematis plants was established. The optimum concentrations of BAP and zeatin in the culture medium regulating in vitro morphogenetic processes in clematis explants cv. Serenada Kryma were determined. Molecular-genetic analysis of Clematis plants obtained via in vitro somatic embryogenesis and organogenesis was carried out. Using ISSR primers 105 amplicons have been revealed, six of them were polymorphic. The mean index of heterogeneity of clematis plants was 5,7 %.