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Lukash L. L., Lylo V. V., Man’ko V. G., Terentev A. G.
The Increasing Mutagenic Effect of Nitrosoguanidine under Influence of Modified Bases during Inhibition of Repair AGT Enzyme in Mammalian Somatic Cells in vitro
Cytology and Genetics 2002, v 36, number 3, 41-50
Gene mutations were studied on human cells SL68, XP12BE and chinese hamster cells Blld-ii-FAF28C1237. All the cells were sensitive to purine base analogs and were characterized by a high rate of O6-alkylguanine-DNA-transferase (AGT) activity. Inhibiting AGT activity by O6-benzylguanine considerably increases the frequency of mutants induced by the alkylating agent MNNG. Transitions of the GC→AT type are the dominant mutations in the coding region of the hprt gene. The mechanism of DNA lesion repair by the AGT enzyme differs significantly from the excision repair.
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