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Evtushenko D. P., Shakhovskiy A. M., Sidorov V. A.
Production of Potato Cybrids without Using Genetic Selectable Markers of the Parental Material: Solanum tuberosum L. Plants (cv. Svitanok Kyivsky) with S. pinnatisectum Dun. Plastids
Cytology and Genetics 2001, v 35, number 6, 3-9
Interspecific cybrid plants of potato were created using the cell technology that does not require the presence of any genetic selectable markers in the parental material. Cybrid production was based on double inactivation of S. pinnatisectum Dun. protoplasts, served as the donors of organelles (by γ-irradiation for the nuclei inactivation and by chemical mutagenesis for the efficient induction of chlDNA mutations), and the transfer of mutant plastids into S. tuberosum L. by protoplast fusion. Selection of cell colonies for streptomycin resistance was performed to identify the cybrid clones with mutant donor-type plastids. Restriction analysis of organelle DNA, chromosome counting, and isoenzyme analysis of the cybrids revealed the presence of nuclear material of S. tuberosum L. (cv. Svitanok kyivsky) and plastids from wild tuber-bearing S. pinnatisectum Dun. These plants enable the study of traits encoded by organelle DNA and to broaden the cytoplasmic diversity of cultivated potato.
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