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Application of the RAPD-Method for Creation of DNA-Markers to Vrn Genes

Balashova I.A., Sivolap Yu.M., Fait V.I., Stelmakh A.F.

 




The possibility of using the PCR with arbitrary primers to mark Vrn genes, Vrn3 in particular, was investigated. RAPD-analysis of DNA of near isogenic lines on Vrn1, Vrn2 and Vrn3 loci in genefone of four varieties of winter wheat were carried out. Monogenic dominant lines by Vrn3 locus possessed 657 b.p. polymorphic DNA fragment. The possibility of using this polymorphic DNA amplicon as a molecular marker was confirmed by results of RAPD-analysis of DNA of some commercial wheat varieties of different ecological-geographic origin possessing dominant Vrn3 alleles, as well as by RAPD-analysis of F2 population from cross of monogenic Vrn3 dominant spring line with its recurrent parent — winter wheat variety Mironovskaya 808. The results of RAPD are comparable with hybridological analysis data concerning type of development of plants from F2 population.

Tsitologiya i Genetika 2001, vol. 35, no. 2, pp. 49-53



Balashova I.A., Sivolap Yu.M., Fait V.I., Stelmakh A.F. Application of the RAPD-Method for Creation of DNA-Markers to Vrn Genes, Tsitol Genet., 2001, vol. 35, no. 2, pp. 49-53.




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