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The immunophenotypic and cytogenetic characteristics of the blast cells in subvariants of acute myeloid leukemia

Isakova L.M., Tretiak N.N., Khromiak V.N., Gordienko A.I., Zverkova A.S., Vakulchuk A.M.

 




The results of study of morpho-cytochemical peculiarities, antigenic profile and peripheric blood and/or bone marrow blast cell karyotype of 21 patients with acute myeloid leukemia are presented. Hemopoietic cell immunophenotyping was carried out with the use of cytofluorometer FACScan and chromosome cytogenetic analysis with the use of analyzer "Metascan". It has been shown that in the AML M1 blast cell plasmatic membrane carries pan-myeloid CD33 and CD13 antigens, the last having high density of expression, and the CD38 antigen, which is a myeloid cell-precursor marker. In these patients tetraploidy, being the testimony of karyotype change, has been ascertained. It has been found out that the AML M2 blasts, except pan-myeloid antigens, express the cell proliferation CD71 marker. Blast cell karyotype peculiarities typical for this leukemia sub-variant have been revealed. In patients with the AML M4 in 3 of 6 cases an anomalous karyotype has been found. It has been also shown that the CD14 antigen, and rather its percentage in blast total population, is the differential-diagnostic criterion for the AML M4 and M5.

Tsitologiya i Genetika 2000, vol. 34, no. 1, pp. 48-56



Isakova L.M., Tretiak N.N., Khromiak V.N., Gordienko A.I., Zverkova A.S., Vakulchuk A.M. The immunophenotypic and cytogenetic characteristics of the blast cells in subvariants of acute myeloid leukemia, Tsitol Genet., 2000, vol. 34, no. 1, pp. 48-56.




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