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The construction and use of inosine-containing primers for searching for and identifying the genes of insecticidal crystal proteins in Bacillus thuringiensis

Rymar’ S.E., Isakova I.A., Kirilenko S.D., Kordium V.A.

 




The specific to 3 types of Cry genes primers containing inosine were constructed to find crystal insecticidal protein Bacillus thuringiensis genes using PCR. A number of new B. thuringiensis strains isolated in Ukraine were investigated using these PCR primers. As a results, Cry genes were found, some of them were identified and demonstrated high homology to Cry1Ba2 and Cry1Bc genes.

Tsitologiya i Genetika 1999, vol. 33, no. 2, pp. 74-78



Rymar’ S.E., Isakova I.A., Kirilenko S.D., Kordium V.A. The construction and use of inosine-containing primers for searching for and identifying the genes of insecticidal crystal proteins in Bacillus thuringiensis, Tsitol Genet., 1999, vol. 33, no. 2, pp. 74-78.




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