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The evaluation of the cytotoxicity of preparations with anticarcinogenic action in human cell cultures

Lukash LL, Paton EB, Sukhorada EM, Ruban TA, Krupskaia IS, Kostetskaia EV, Bratsiuk SI

 




An approach for determination of cytotoxicity of chemical and biological drugs which combine the estimation of quality and quantity of cells after their treatment are proposed by the authors. The visual control of cellular minicultures permits one: 1) to register specific and pathologic alterations of cell morphology, 2) to trace their development in dynamics under the action of individual or complex drugs and 3) to choose the optimum time for the experiment fixation. The special staining of the treated cells and measurement of the optical density of absorbed histological dye permits one to make a conclusion about the changing of the cells quantity. A combined method of double estimation gives the opportunity to detect the artefacts taking place after staining the cells treated by some drugs and extracts of natural origin in high concentrations.

Tsitologiya i Genetika 1997, vol. 31, no. 6, pp. 26-34



Lukash LL, Paton EB, Sukhorada EM, Ruban TA, Krupskaia IS, Kostetskaia EV, Bratsiuk SI The evaluation of the cytotoxicity of preparations with anticarcinogenic action in human cell cultures, Tsitol Genet., 1997, vol. 31, no. 6, pp. 26-34.




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