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The genetic transformation of Lycopersicon peruvianum var. dentatum by using Agrobacterium tumefaciens carrying a plasmid with the human beta-interferon gene

Rudas VA, Piven’ NM, Rivkin MI, Vershinin AV, Infante DC

 




Wild tomato plants of Lycopersicon peruvianum var. dentatum were transformed with pG11 plasmid having human beta-interferon (hIFN-beta) and NPTII genes. Transformation was demonstrated by polymerase chain reaction (PCR) and ELISA assay. Expression of hIFN-beta gene was identified by Western blot analysis. Transgenic plants produced seeds and F1 generation inherited integrated traits.

Tsitologiya i Genetika 1997, vol. 31, no. 2, pp. 17-22



Rudas VA, Piven’ NM, Rivkin MI, Vershinin AV, Infante DC The genetic transformation of Lycopersicon peruvianum var. dentatum by using Agrobacterium tumefaciens carrying a plasmid with the human beta-interferon gene, Tsitol Genet., 1997, vol. 31, no. 2, pp. 17-22.




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